Each of the four papal basilicas in Rome (St Peter's in the Vatican, St John Lateran, St Mary Major and St Paul Outside the Walls) has a Holy Door. These are jubilee churches, where pilgrims can go on pilgrimage to obtain an indulgence by fulfilling the conditions. The Basilicas of the Holy Land are also traditionally jubilee churches. In the rest of the world, churches and any shrines where each local Ordinary has ordered the opening of a Door of Mercy are to be considered jubilee churches.

In the case of Rome, the four Papal Basilicas are joined by the three churches that, together with them, make up the traditional itinerary of the 'seven churches', namely San Lorenzo fuori le Mura, Santa Croce in Gerusalemme and San Sebastiano fuori le Mura. The importance of the Jubilee pilgrimage suggests that this penitential itinerary left to the Romans by St Philip Neri in the 16th century should be rediscovered and practised. Therefore, the pilgrimage to each of these churches will also be an opportunity to experience the Jubilee indulgence.

The diocese of Rome is also home to many other important churches and shrines, pilgrimage destinations for many believers who always find priests there ready to welcome them into the Father's mercy. During the Holy Year, the Sanctuary of Divine Love and the Church of Santo Spirito in Sassia, also known as the 'Sanctuary of Divine Mercy', will also be jubilee churches, where pilgrims can go on pilgrimage to obtain indulgences.

Finally, the churches that already permanently enjoy the privilege of indulgence for the faithful who devoutly fulfil the conditions laid down, will continue to be places where the indulgence can be obtained also during the Jubilee of Mercy, according to what has already been determined for each of these places.